A practical introduction to molecular methods, sample preparation and laboratory automation.
Real-time PCR monitors amplification through fluorescence. Assay targets, channels, controls and intended use must be checked in the current manufacturer instructions. A shared method does not establish compatibility between products.
Extraction isolates nucleic acids from a sample before downstream analysis. Sample type, input volume, elution and inhibitor removal depend on the validated protocol. Review the specific kit and instrument combination.
Consider batch sizes, hands-on steps, space and service requirements when evaluating instruments. Request documented compatibility and training requirements for your intended application.
Use controls and acceptance criteria specified in the manufacturer instructions and your laboratory procedures. Contact our team with your target, sample type and instrument model to request relevant documentation.